The newer tests are not refrigerated, you will now get a dry well that needs to be re hydrated. If you do not use proper pipetting technique you will NOT have enough diluent to use in all 25 tests! Also, if you use improper pipetting technique your test results will NOT be accurate! Each bottle of diluent contains approximately 4900ul of fluid, each test requires 150ul of fluid, 150ul X 25 tests equals 3750ul, therefor each bottle of diluent contains enough fluid to perform 32 tests so there is ample fluid. If you are coming up short on diluent you are not using your pipette correctly or your pipette needs to be re calibrated. Please view the videos to be sure you are performing all the steps properly.